Question

A nucleic acid fragment is added to four dif- ferent tubes along with a polymerase, a radio- labeled primer, and deoxynucleotides. Each tube also contains one of the four bases as dideoxynucleotides. The four tubes are then run on electrophoresis gel and visualized by autoradiography. For which of the following purposes would the described laboratory tech- nique be utilized?

1. (A)  To amplify DNA fragments

2. (B)  To create an allele-specific oligonucleotide

   probe

3. (C)  To decipher the order of nitrogenous bases

   in the human genome

4. (D)  To determine the base pairing of a seg-

   ment of DNA with a DNA probe

5. (E)  To determine the base pairing of a seg-

   ment of RNA with a DNA probe

6. (F)  To establish the presence of a given pro-

   tein

7. (G)  To show the presence of a specific anti-

   body in plasma