A patient who is a carrier of sickle cell trait presents to the clinic. The single base-pair mu- tation for sickle cell anemia destroys the MstII restriction enzyme recognition site represented by an asterisk in the image. The restriction enzyme-binding sites are shown as arrows on the map. DNA from this patient is treated with MstII and run on an electrophoresis gel. The DNA is then hybridized with a labeled probe that binds to the normal gene in the posi- tion shown on the map. In the Southern blot shown in the image, which lane represents the patient?

Question

A patient who is a carrier of sickle cell trait presents to the clinic. The single base-pair mu- tation for sickle cell anemia destroys the MstII restriction enzyme recognition site represented by an asterisk in the image. The restriction enzyme-binding sites are shown as arrows on the map. DNA from this patient is treated with MstII and run on an electrophoresis gel. The DNA is then hybridized with a labeled probe that binds to the normal gene in the posi- tion shown on the map. In the Southern blot shown in the image, which lane represents the patient?

by admin (2.6k points) in Biochemistry asked Sep 13

(A) A

(B) B

(C) C

(D) D

(E) E

(F) F

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