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A segment of DNA is isolated and added to a mixture of four deoxynucleotides, two specific oligonucleotide sequences, and heat-stable DNA polymerase. The mixture is then heated to denature the DNA, cooled, and reheated in a number of cycles. Which of the following laboratory techniques does this describe?

(A) Enzyme-linked immunosorbent assay

 (B) Gel electrophoresis
(C) Northern blot
(D) Polymerase chain reaction

(E) Sequencing

 (F) Southern blot

 (G) Western blot

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